CV
Education
- M.Sc. in Molecular Science, Ryerson University, 2017
- Dissertation: “The PH domain from the Toxoplasma gondii PH-containing protein-1 (TgPH1) serves as an ectopic reporter of phosphatidylinositol 3-phosphate in mammalian cells “
- B.Sc. in Microbiology, University of Guelph, 2013
Work experience
- Research Associate (Xtal Biostructures Inc.),Apr 2019 – Present Natick, MA, U.S.A.
- Purified several proteins for clients using FPLCs (Biorad Biologic LPs and AKTA)
- Performed thermal shift assay(DSF) including the completion of a project with 1700 compounds
- Utilized SPR for clients in order to identify molecules with desirable binding parameters
- Assisted crystallographers in setting up trays for X-ray crystallography
- Private Tutor, Aug 2018 – Dec 2018, Toronto, Canada
- Provided homework help and tutoring to Grade 11 and 12 students in Physics and Math
- Feb 2018 - Apr 2018: Technician, Botelho Lab, Ryerson University
- Cataloged existing chemicals and inventory into a new ordering system (Quartzy) to maximize efficiency and productivity in two months
- Liaised with the facilities manager on keeping Good Laboratory Practices (GLP) and Standard Operating Procedures (SOP) ensuring compliance by lab
- 2013 - 2017: Graduate Research Assistant, Botelho Lab, Ryerson University
- Characterized the protein TgPH1 as an ectopic reporter for endosomal phosphoinositides after fluorescent tagging and authored project findings in a first author publication in PLOS ONE
- Characterized recombinant Vac14 interactions using immunoprecipitation and pull-down assays post expression and purification from yeast and bacteria
- Guided team goals by monitoring project outcomes, communicated and collaborated regularly with others to meet deadlines to complete experiments for publications
- 2013 - 2017: Graduate Teaching Assistant, Department of Chemistry and Biology, Ryerson University
- Demonstrated procedures and instrument use to weekly undergraduate lab sections
- Graded and provided feedback to student lab reports and presentations
- 2013 - 2013: Research Assistant, Meng Lab, University of Guelph
- Successfully expressed and purified the coat protein of Grapevine Rupestris stem pitting-associated virus (GRSPaV) after cloning into the PET28a vector system
- Identified viral infection in Begonia hiemalis plants by Impatiens Necrotic Spot Virus (INSV) with results published in the Plant Disease journal
Skills
- Common Techniques in Bacteriology and Mammalian Cell Culture
- Nucleic Acid Extraction and Manipulation (Gel Electrophoresis, RE Digestion, PCR, qPCR)
- Gene Synthesis and Primer Design
- Molecular Cloning
- In Silico Analysis(DNA/Protein)
- Microscopy (Fluorescence and Spinning Disk Confocal)
- Yeast/Bacterial Protein Expression and Purification
- Protein Analysis (SDS-PAGE, Western Blot
- Immunoprecipitation and Pull-Down Assay
- Experimental Design and Statistical Analysis
Publications
Talks
In the hunt for a novel probe for phosphatidylinositol 3,5-bisphosphate
Conference Proceedings Poster Presentation at Canadian Society of Molecular Biosciences Conferece 2017, Ottawa, Canada
Teaching
Service and leadership
- Currently signed in to 43 different slack teams